Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNG1/Kv6.1 (extracellular) Blocking Peptide (#BLP-PC135) is the original antigen used for immunization during Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135) generation. The blocking peptide binds and ‘blocks’ Anti-KCNG1/Kv6.1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135), (1:400).
3,4. Anti-KCNG1 (KV6.1) (extracellular) Antibody, preincubated with KCNG1/Kv6.1 (extracellular) Blocking Peptide (#BLP-PC135).
Expression of KV6.1 potassium channel in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135), (1:200). A. KV6.1 is detected in rat cerebellum (red). Staining is apparent in Purkinje neurons (arrow) and in the molecular layer (Mol). B. Cell nuclei in the same section are stained with DAPI (blue). C. Merge of the two images.
Properties
Sequence
- (C)RRKPSTGNSYLDK, corresponding to amino acid residues 325-337 of mouse KV6.1 (Accession A2BDX4).
Accession (Uniprot) Number A2BDX4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
