Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNH2/erg1 Blocking Peptide (#BLP-PC016) is the original antigen used for immunization during Anti-KCNH2 (erg1) Antibody (#APC-016) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH2/erg1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of HEK 293 cell lysate, stably expressing HERG channels:1. Anti-KCNH2 (erg1) Antibody (1:200).
2. Anti-KCNH2 (erg1) Antibody, preincubated with KCNH2/erg1 Blocking Peptide (#BLP-PC016).- Mouse heart sections.
Properties
Sequence
- (CY)EEL PAGAP ELPQD GPT, corresponding to residues 1122-1137 of rat KV11.1 (erg1) (Accession O08962).
Accession (Uniprot) Number O08962
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
