Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNH2/HERG (extracellular) Blocking Peptide (#BLP-PC109) is the original antigen used for immunization during Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH2/HERG (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of KV11.1 (HERG)-transfected HEK-293 cells:1. Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109), (1:200).
2. Anti-KCNH2 (HERG) (extracellular) Antibody, preincubated with KCNH2/HERG (extracellular) Blocking Peptide (#BLP-PC109).
Expression of KV11.1 (HERG) in rat heartImmunohistochemical staining of rat heart using Anti-KCNH2 (HERG) (extracellular) Antibody (#APC-109). A. Transversal section of the atrium wall; note that arterial smooth muscle fibers were not stained (green arrow). B. Longitudinal section of the myocardium. C. Section showing myocardium and endocardium (red arrow). DAB product is brown and the counterstain is cresyl violet.
Properties
Sequence
- AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1 (HERG) (Accession Q12809).
Accession (Uniprot) Number Q12809
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
