Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNH5/EAG-2 Blocking Peptide (#BLP-PC053) is the original antigen used for immunization during Anti-KCNH5 (EAG-2) Antibody (#APC-053) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH5/EAG-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNH5 (EAG-2) Antibody (#APC-053), (1:200).
2. Anti-KCNH5 (EAG-2) Antibody, preincubated with KCNH5/EAG-2 Blocking Peptide (#BLP-PC053).- Rat retina (Jow, G.M. and Jeng, C.J. (2008) Neurosci. Lett. 431, 12.).
Properties
Sequence
- (C)EDPKGSDSENSVTKNPLRK, corresponding to amino acid residues 842-860 of rat KCNH5 (Accession Q9EPI9).
Accession (Uniprot) Number Q9EPI9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
