Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNH6/erg2 Blocking Peptide (#BLP-PC114) is the original antigen used for immunization during Anti-KCNH6 (erg2) Antibody (#APC-114) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH6/erg2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (1 and 3) and cortex (2 and 4) lysate:1,2. Anti-KCNH6 (erg2) Antibody (#APC-114), (1:200).
3,4. Anti-KCNH6 (erg2) Antibody, preincubated with KCNH6/erg2 Blocking Peptide (#BLP-PC114).
Expression of KV11.2 (erg2) in rat hippocampusImmunohistochemical staining of KV11.2 in rat hippocampus using Anti-KCNH6 (erg2) Antibody (#APC-114). A. KV11.2 appears as diffuse staining (green) that defines the boundary of the reticular nucleus of thalamus (arrow). B. Staining with mouse anti-parvalbumin (PV, red) demonstrates both neuronal and diffuse staining. C. Confocal merge of KV11.2 and PV demonstrates overlap of the two markers labeling the reticular nucleus of the thalamus (orange).- Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
Properties
Sequence
- TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human KV11.2 (erg2) (Accession Q9H252).
Accession (Uniprot) Number Q9H252
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
