Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058) is the original antigen used for immunization during Anti-KCNJ15 (Kir4.2) Antibody (#APC-058) generation. The blocking peptide binds and ‘blocks’ Anti-KCNJ15/Kir4.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058), (1:200).
2. Anti-KCNJ15 (Kir4.2) Antibody, preincubated with KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058).- Rat retina sections (1:100) (Tian, M. et al. (2003) Neurosci. Lett. 345, 9.).
Properties
Sequence
- (C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse KCNJ15 (Accession O88932).
Accession (Uniprot) Number O88932
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
