Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNJ5/Kir3.4 Blocking Peptide (#BLP-PC027) is the original antigen used for immunization during Anti-KCNJ5 (Kir3.4) Antibody (#APC-027) generation. The blocking peptide binds and ‘blocks’ Anti-KCNJ5/Kir3.4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-KCNJ5 (Kir3.4) Antibody (#APC-027), (1:200).
2. Anti-KCNJ5 (Kir3.4) Antibody, preincubated with KCNJ5/Kir3.4 Blocking Peptide (#BLP-PC027).- Rat heart sections (1:20) (Atkinson, A.J. et al. (2013) J. Am. Heart Assoc. 2, e000246.).
Properties
Sequence
- RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4 (Accession P48548).
Accession (Uniprot) Number P48548
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
