Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110) is the original antigen used for immunization during Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK1/TWIK-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:200).
2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110).
Western blot analysis of HEK-293-KCNK1 transfected cells:1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:300).
2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110).
Expression of KCNK1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110). A. KCNK1 channel appears in glial processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK1 channel and calbindin D28K demonstrates the separate localization of these proteins.
Properties
Sequence
- RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 (Accession O00180).
Accession (Uniprot) Number O00180
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
