Cat #: BLP-PC110
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 (Accession O00180).
Accession (Uniprot) Number O00180
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysate:1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:200).
2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110). - Western blot analysis of HEK-293-KCNK1 transfected cells:1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:300).
2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110). - Rat brain sections.
Last update: 02/01/2024