Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169) is the original antigen used for immunization during Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK12/THIK-2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1,2. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:400).
3,4. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):1,2. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:200).
3,4. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:200).
2. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
Expression of KCNK12 (THIK-2) in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:400), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. THIK-2 immunoreactivity (green) in the rat hippocampal CA3 region is detected in neuronal soma (horizontal arrows) and in the dendrite initial segment (vertical arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)ESPGEAEARARWG, corresponding to amino acid residues 61-73 of rat KCNK12 (Accession Q9ERS1).
Accession (Uniprot) Number Q9ERS1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
