Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)ESPGEAEARARWG, corresponding to amino acid residues 61-73 of rat KCNK12 (Accession Q9ERS1).
Accession (Uniprot) Number Q9ERS1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1,2. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:400).
3,4. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):1,2. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:200).
3,4. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:200).
2. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).- Rat brain sections (1:400).
Last update: 02/01/2024
