Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16 (Accession Q96T55).
Accession (Uniprot) Number Q96T55
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas lysate:1. Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170), (1:600).
2. Anti-KCNK16 (TALK-1) (extracellular) Antibody, preincubated with KCNK16/TALK-1 (extracellular) Blocking Peptide (#BLP-PC170).
Western blot analysis of human PANC-1 pancreas ductal adenocarcinoma cell line lysate:1. Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170), (1:600).
2. Anti-KCNK16 (TALK-1) (extracellular) Antibody, preincubated with KCNK16/TALK-1 (extracellular) Blocking Peptide (#BLP-PC170).
Expression of KCNK16 (TALK-1) in mouse pancreas.Immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) mouse pancreas sections, with Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170), (1:150), followed by goat anti-rabbit-AlexaFluor-647. A. TALK-1 immunoreactivity (red) appears in islets of Langerhans. B. Pre-incubation of the antibody with KCNK16 (TALK-1) (extracellular) Blocking Peptide (#BLP-PC170), suppressed staining. Cell nuclei are stained with DAPI (blue). Heat-induced epitope retrieval was done with citrate buffer, pH6.
Last update: 02/01/2024
