Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122) is the original antigen used for immunization during Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK18/TRESK (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes:1,2. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:200).
3,4. Anti-KCNK18 (TRESK) (extracellular) Antibody, preincubated with KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122).
Western blot analysis of mouse brain membranes:1. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:200).
2. Anti-KCNK18 (TRESK) (extracellular) Antibody, preincubated with KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122).
Expression of KCNK18 in rat dorsal root gangliaImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:100) followed by Alexa 555-labeled secondary antibody (red staining). Both big/medium sized neurons and small neurons are stained. Note that glial cells and axonal fibers are not stained. Hoechst 33342 is used as the counterstain (blue staining).
Properties
Sequence
- (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18 (Accession Q6VV64).
Accession (Uniprot) Number Q6VV64
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
