Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK2/TREK-1 Blocking Peptide (#BLP-PC047) is the original antigen used for immunization during Anti-KCNK2 (TREK-1) Antibody (#APC-047) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK2/TREK-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNK2 (TREK-1) Antibody (#APC-047), (1:200).
2. Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).- Rat hippocampus sections (Banerjee, A. et al. (2016) J. Neurochem. 138, 265.).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:800).
3,4. Guinea pig Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).
Expression of KCNK2 (TREK-1) in rat midbrainImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:400), followed by goat-anti-guinea pig-Cy3 antibody. TREK-1 staining (red) appears in neurons in the rat lateral substantia nigra (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069).
Accession (Uniprot) Number O95069
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
