Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK3/TASK-1 Blocking Peptide (#BLP-PC024) is the original antigen used for immunization during Anti-KCNK3 (TASK-1) Antibody (#APC-024) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK3/TASK-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (1, 3) and pancreas (2, 4) membranes:1,2. Anti-KCNK3 (TASK-1) Antibody (#APC-024), (1:200).
3,4. Anti-KCNK3 (TASK-1) Antibody, preincubated with KCNK3/TASK-1 Blocking Peptide (#BLP-PC024).- Rat brain sections.
Properties
Sequence
- (C)EDEKRDAEHRALLTRNGQ, corresponding to amino acid residues 252-269 of human KCNK3 (Accession O14649).
Accession (Uniprot) Number O14649
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
