Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK4/TRAAK Blocking Peptide (#BLP-PC108) is the original antigen used for immunization during Anti-KCNK4 (TRAAK) Antibody (#APC-108) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK4/TRAAK primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of HEK-293-KCNK4 transfected cells:1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).
Western blot analysis of rat cerebellum lysate:1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).
2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).
Expression of KCNK4 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-KCNK4 (TRAAK) Antibody (#APC-108 ). A. KCNK4 channel appears in Purkinje neuronal processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK4 channel and calbindin D28K demonstrates the co-localization of these proteins.
Properties
Sequence
- NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 (Accession Q9NYG8).
Accession (Uniprot) Number Q9NYG8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
