Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK5/TASK-2 Blocking Peptide (#BLP-PC037) is the original antigen used for immunization during Anti-KCNK5 (TASK-2) Antibody (#APC-037) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK5/TASK-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:200).
2. Anti-KCNK5 (TASK-2) Antibody, preincubated with KCNK5/TASK-2 Blocking Peptide (#BLP-PC037).
Expression of KCNK5 in rat pancreasImmunohistochemical staining of rat pancreas was visualized using Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Substrate (Histofine, Nichirei Corp.) was used for the color reaction. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human KCNK5 (Accession O95279).
Accession (Uniprot) Number O95279
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
