Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNK9/TASK-3 (extracellular) Blocking Peptide (#BLP-PC044) is the original antigen used for immunization during Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK9/TASK-3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of KCNK9 in rat cerebellum (lanes 1, 4), human mammary gland MCF-10A (lanes 2, 5) and MCF-7 (lanes 3, 6) cell lysates (upper panel):1-3. Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044).
4-6. Anti-KCNK9 (TASK-3) (extracellular) Antibody, preincubated with KCNK9/TASK-3 (extracellular) Blocking Peptide (#BLP-PC044).
Lower panel: anti-β actin to ensure equal loading.
Note that the expected 50 kD MW band is present in cerebellum and in the tumorigenic breast cancer cell line (MCF-7) but not in the non-tumorigenic breast cell line (MCF-10A).
Western blot analysis of rat cerebellum lysate:1. Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044), (1:200).
2. Anti-KCNK9 (TASK-3) (extracellular) Antibody, preincubated with the control peptide antigen.- Rat brain sections (Okamoto, K. et al. (2016) eNeuro 3, e0138-16.2016.).
Properties
Sequence
- (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat KCNK9 (Accession Q9ES08).
Accession (Uniprot) Number Q9ES08
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
