Overview
Type: GST fusion protein
Form: Lyophilized powder
KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021) is the original antigen used for immunization during Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#APC-021) generation. The blocking peptide binds and ‘blocks’ Anti-KCNMA1/KCa1.1 (1097-1196) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#APC-021), (1:200).
2. Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody, preincubated with KCNMA1 (KCa1.1) (1097-1196) Blocking Peptide (#BLP-PC021).- Rat brain sections.
Mouse vomeronasal sections (Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824.). 
Western blot analysis of rat bladder lysate:1. Guinea pig Anti-KCNMA1 (KCa1.1) (1097–1096) Antibody (#APC-021-GP), (1:200).
2. Guinea pig Anti-KCNMA1 (KCa1.1) (1097–1096) Antibody, preincubated with KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021).
Western blot analysis of mouse brain membranes:1. Guinea pig Anti-KCNMA1 (KCa1.1) (1097–1096) Antibody (#APC-021-GP), (1:200).
2. Guinea pig Anti-KCNMA1 (KCa1.1) (1097–1096) Antibody, preincubated with KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021).
Western blot analysis of human Colo205 colon adenocarcinoma (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):1,2. Guinea pig Anti-KCNMA1 (KCa1.1) (1097–1096) Antibody (#APC-021-GP), (1:200).
3,4. Guinea pig Anti-KCNMA1 (KCa1.1) (1097–1096) Antibody, preincubated with KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021).
Properties
Sequence
- SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2 (Accession Q08460-2).
Accession (Uniprot) Number Q08460-2
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 1.2 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg fusion protein per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
