Name: KCNMA1/KCa1.1 (1097-1196) Blocking Peptide
Brand: Alomone Labs
SKU: BLP-PC021

Back to product page SDS

Cat #: BLP-PC021

Size: 0.12 mg

Type: GST fusion protein

Form: Lyophilized powder

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Properties

Sequence

SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2 (Accession Q08460-2).

Accession (Uniprot) Number Q08460-2

Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.

Purity >95% (SDS-PAGE)

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 μl PBS.

Concentration After Reconstitution 1.2 mg/ml.

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 3 µg fusion protein per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat brain membranes:
1. Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody (#APC-021), (1:200).
2. Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody, preincubated with KCNMA1 (K_Ca1.1) (1097-1196) Blocking Peptide (#BLP-PC021).
Rat brain sections.

Mouse vomeronasal sections (Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824.).
Western blot analysis of rat bladder lysate:
1. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097–1096) Antibody (#APC-021-GP), (1:200).
2. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097–1096) Antibody, preincubated with KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021).
Western blot analysis of mouse brain membranes:
1. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097–1096) Antibody (#APC-021-GP), (1:200).
2. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097–1096) Antibody, preincubated with KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021).
Western blot analysis of human Colo205 colon adenocarcinoma (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):
1,2. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097–1096) Antibody (#APC-021-GP), (1:200).
3,4. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097–1096) Antibody, preincubated with KCNMA1/KCa1.1 (1097-1196) Blocking Peptide (#BLP-PC021).

Last update: 02/01/2024

Specific Use it with:

Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody (#APC-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNMA1 from human, mouse, and rat samples.

Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody (#APC-021)
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse KCNMA1 channel. Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody (#APC-021-GP, formerly AGP-014), raised in guinea pigs, can be used in western blot, immunoprecipitation, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KCNMA1 from human, mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody (#APC-021) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Guinea pig Anti-KCNMA1 (K_Ca1.1) (1097-1196) Antibody (#APC-021-GP)