Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNMA1/KCa1.1 (1184-1200) Blocking Peptide (#BLP-PC107) is the original antigen used for immunization during Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) generation. The blocking peptide binds and ‘blocks’ Anti-KCNMA1/KCa1.1 (1184-1200) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107), (1:500).
2. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody, preincubated with KCNMA1/KCa1.1 (1184-1200) Blocking Peptide (#BLP-PC107).
Expression of KCNMA1 in rat penisImmunohistochemical staining of rat penis transversal section using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107). Strong and specific immunostaining is evident in both corpus cavernosum smooth muscle cells (blue arrow) and in the muscular layer of the penis artery (green arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Subtrate (histofine, Nichirei Corp.) was used for the colour reaction. Hematoxillin is used as the counterstain.
Properties
Sequence
- (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCNMA1 (Accession Q08460).
Accession (Uniprot) Number Q08460
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
