Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNN2/KCa2.2 Blocking Peptide (#BLP-PC028) is the original antigen used for immunization during Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028) generation. The blocking peptide binds and ‘blocks’ Anti-KCNN2/KCa2.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNN2 (KCa2.2, SK2) Antibody #APC-028), (1:200).
2. Anti-KCNN2 (KCa2.2, SK2) Antibody, preincubated with KCNN2/KCa2.2 Blocking Peptide (#BLP-PC028).
Expression of KCNN2 (SK2) in rat cerebral cortexImmunohistochemical staining of KCNN2 channels using Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028) in rat cerebral cortex. Picture showing the the third layer. Note that both neural (black arrows) and glial cells (green arrows) show intense staining. Reaction product is red and counterstain is H&E.
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028-GP), (1:1000).
3,4. Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody, preincubated with KCNN2/KCa2.2 Blocking Peptide (#BLP-PC028).
Properties
Sequence
- (C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542-559 of rat KCNN2 (Accession P70604).
Accession (Uniprot) Number P70604
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
