Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNN3/KCa2.3 (N-term) Blocking Peptide (#BLP-PC025) is the original antigen used for immunization during Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025) generation. The blocking peptide binds and ‘blocks’ Anti-KCNN3/KCa2.3 (N-term) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025), (1:200).
2. Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody, preincubated with KCNN3/KCa2.3 (N-term) Blocking Peptide (#BLP-PC025).
Expression of KCa2.3 (SK3) in mouse dopaminergic neuronsImmunohistochemical staining of mouse dopaminergic neurons using Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025). A. KCa2.3 is detected in substantia nigra pars compacta. B. Tyrosine hydroxylase staining shows dopaminergic neurons. Triangles point at cells with co-localization.
Properties
Sequence
- DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCNN3 (Accession Q9UGI6).
Accession (Uniprot) Number Q9UGI6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
