Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNN4/KCa3.1 Blocking Peptide (#BLP-PC064) is the original antigen used for immunization during Anti-KCNN4 (KCa3.1, SK4) Antibody (#APC-064) generation. The blocking peptide binds and ‘blocks’ Anti-KCNN4/KCa3.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of KCa3.1-transfected HEK-293 (lanes 1 and 3) and Human K562 chronic myelogenous leukemia (lanes 2 and 4) cells:1,2. Anti-KCNN4 (KCa3.1, SK4) Antibody (#APC-064), (1:200).
3,4. Anti-KCNN4 (KCa3.1, SK4) Antibody, preincubated with KCNN4/KCa3.1 Blocking Peptide (#BLP-PC064).- Rat mesenteric artery parafin-embedded sections (Hilgers, R.H. et al. (2010) J. Pharmacol. Exp. Ther. 333, 210.).
Properties
Sequence
- RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4 (Accession Q9QYW1).
Accession (Uniprot) Number Q9QYW1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
