Cat #: ESB-503_PACK
Size: 14 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-KCNQ1 Antibody Cat #: APC-022
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787). Intracellular, C-terminus.
Homology
Rat, mouse - 14/16 amino acid residues identical.
Anti-KCNQ2 Antibody Cat #: APC-050
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession O88943). Intracellular, C-terminus.
Homology
Mouse - identical; human - 15/16 amino acid residues identical.
Anti-KCNQ3 Antibody Cat #: APC-051
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
M, R
Immunogen
Peptide AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3 (Accession O88944). Intracellular, C-terminal.
Homology
Mouse - identical; human - 18/19 amino acid residues identical.
Anti-KCNQ4 Antibody Cat #: APC-164
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)DRKTREKGDKGPSD, corresponding to amino acid residues 594-607 of mouse KCNQ4 (Accession Q9JK97). Intracellular, C-terminus.
Homology
Rat - identical; human - 12/14 amino acid residues identical.
Anti-KCNQ5 Antibody Cat #: APC-155
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RGSQDFYPKWRESK, corresponding to amino acid residues 856-869 of mouse KCNQ5 (Accession Q9JK45). Intracellular, C-terminus.
Homology
Human, rat - identical.
Pharmacological Reagents
2,5-dimethylcelecoxib Cat #: D-150
Size:
25 mg
Source: Synthetic
MW: 395.4
Purity: >99%
Effective concentration
10 µM.
Activity
2,5-dimethylcelecoxib is a KCNQ channel opener in smooth muscle cells1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Linopirdine dihydrochloride Cat #: L-156
Size:
25 mg
Source: Synthetic
MW: 464.39
Purity: >98%
Effective concentration
0.1-300 µM.
Activity
Linopirdine blocks KCNQ channels with an IC50 of 2.4 μM for KV7.2/3 heteromers1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in water. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
XE991 dihydrochloride Cat #: X-101
Size:
25 mg
Source: Synthetic
MW: 449.37
Purity: >98%
Effective concentration
0.1-300 µM.
Activity
XE991 blocks KCNQ channels with IC50 of 0.5-10 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up to 50mM in water. Mild warming or agitation may be required. Spin-down all product preparations before handling.
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Retigabine dihydrochloride Cat #: R-101
Size:
50 mg
Source: Synthetic
MW: 376.2
Purity: >98%
Effective concentration
0.1-100 µM
Activity
Retigabine is a potent and selective KCNQ (KV7, M-) channel modulator (enhancer)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
For research purposes only, not for human use
