Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNQ1 Blocking Peptide (#BLP-PC022) is the original antigen used for immunization during Anti-KCNQ1 Antibody (#APC-022) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-KCNQ1 Antibody (#APC-022), (1:200).
2. Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).- Mouse distal colon (1:50) (Jepps, T.A. et al. (2009) Am. J. Physiol. 297, G107.).
Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8):1,2,5,7. Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200).
3,4,6,8. Guinea pig Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).
Expression of KCNQ1 in mouse heartImmunohistochemical staining of mouse heart frozen section using Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200). A. KCNQ1 staining (red) appears in muscle fiber membranes (arrows). B. Nuclei are stained using DAPI counterstain (blue). C. Merged image of panels A and B.
Properties
Sequence
- (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787).
Accession (Uniprot) Number P51787
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2.5 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
