Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNQ3 Blocking Peptide (#BLP-PC051) is the original antigen used for immunization during Anti-KCNQ3 Antibody (#APC-051) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KCNQ3 Antibody (#APC-051), (1:200).
2. Anti-KCNQ3 Antibody, preincubated with KCNQ3 Blocking Peptide (#BLP-PC051).
Expression of KCNQ3 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNQ3 Antibody (#APC-051), (1:200), followed by goat anti-rabbit-AlexaFluor-488. KCNQ3 immunoreactivity (green) appears in cells of the pyramidal layer (P, vertical arrows) and cells of the stratum radiatum (SR, horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3 (Accession O88944).
Accession (Uniprot) Number O88944
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
