Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNQ4 Blocking Peptide (#BLP-PC164) is the original antigen used for immunization during Anti-KCNQ4 Antibody (#APC-164) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-KCNQ4 Antibody (#APC-164), (1:200).
3,4. Anti-KCNQ4 Antibody, preincubated with KCNQ4 Blocking Peptide (#BLP-PC164).
Expression of KCNQ4 (KV7.4) in rat lateral dorsal raphe nucleusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KCNQ4 Antibody (#APC-164), (1:400), followed by biotin-labeled anti-rabbit antibody and streptavidin-ATTO Fluor-594 (red). Staining appears in soma of neuronal profiles (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DRKTREKGDKGPSD, corresponding to amino acid residues 594-607 of mouse KCNQ4 (Accession Q9JK97).
Accession (Uniprot) Number Q9JK97
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
