Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNQ5 Blocking Peptide (#BLP-PC155) is the original antigen used for immunization during Anti-KCNQ5 Antibody (#APC-155) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat hippocampus (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:1-3. Anti-KCNQ5 Antibody (#APC-155), (1:200).
4-5. Anti-KCNQ5 Antibody, preincubated with KCNQ5 Blocking Peptide (#BLP-PC155).
Expression of KCNQ5 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNQ5 Antibody (#APC-155), (1:100). A. KCNQ5 (green) is expressed in hilar cells of the hippocampal dentate gyrus. B. Double-staining of KCNQ5 (green) with a neuronal marker (“Milli-Mark” from Millipore, red) reveals partial localization with hilar interneurons (arrow points at an example).
Properties
Sequence
- (C)RGSQDFYPKWRESK, corresponding to amino acid residues 856-869 of mouse KCNQ5 (Accession Q9JK45).
Accession (Uniprot) Number Q9JK45
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
