Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNS3/Kv9.3 (extracellular) Blocking Peptide (#BLP-PC133) is the original antigen used for immunization during Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133) generation. The blocking peptide binds and ‘blocks’ Anti-KCNS3/Kv9.3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4):1,2. Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133), (1:200).
3,4. Anti-KCNS3 (KV9.3) (extracellular) Antibody, preincubated with KCNS3/Kv9.3 (extracellular) Blocking Peptide (#BLP-PC133).
Expression of KV9.3 channel in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133), (1:200). A. KV9.3 staining (red) is expressed in the molecular layer (M). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of KV9.3 expression to the molecular layer.
Properties
Sequence
- (C)EFQNEDGEVDDPVLE, corresponding to amino acid residues 209-223 of rat KCNS3 (Accession O88759).
Accession (Uniprot) Number O88759
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
