Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kidins220 Blocking Peptide (#BLP-PZ017) is the original antigen used for immunization during Anti-Kidins220 Antibody (#APZ-017) generation. The blocking peptide binds and ‘blocks’ Anti-Kidins220 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4):1,2. Anti-Kidins220 Antibody (#APZ-017), (1:200).
3,4. Anti-Kidins220 Antibody, preincubated with Kidins220 Blocking Peptide (#BLP-PZ017).
Expression of Kidins220 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Kidins220 Antibody (#APZ-017), (1:1000), followed by goat-anti-rabbit-AlexaFluor-488. A. Kidins220 staining (green) in hippocampal sections is seen in neurons in the pyramidal layer (Pyr) and stratum oriens (OR) (arrows). B. Staining in cerebellum sections shows labeling (green) in neurons in the Purkinje cell layer (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)PRHSNLSWQSQTRR, corresponding to amino acid residues 1335-1348 of rat Kidins220 (Accession Q9EQG6).
Accession (Uniprot) Number Q9EQG6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
