Cat #: AK-200_KIT
Size: 35 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-KCNJ1 (Kir1.1) Antibody Cat #: APC-001
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence HNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDET DDTQM, corresponding to amino acids 342-391 of rat KCNJ1 (Accession P35560). Intracellular, C-terminus.
Homology
Mouse - identical; human - 45/50 amino acid residues identical.
KCNJ1/Kir1.1 Blocking Peptide Cat #: BLP-PC001
Size:
0.12 mg
Anti-Kir2.1/KCNJ2 Antibody Cat #: APC-026
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1 (Accession P48049). Intracellular, C-terminal part.
Homology
Rabbit, bovine, pig, guinea pig - identical; rat, mouse - 17/19 amino acid residues identical; chicken - 15/19 amino acid residues identical.
Kir2.1/KCNJ2 Blocking Peptide Cat #: BLP-PC026
Size:
40 µg
Anti-Kir2.2 (KCNJ12) Antibody Cat #: APC-042
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.
Homology
Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.
Kir2.2/KCNJ12 Blocking Peptide Cat #: BLP-PC042
Size:
40 µg
Anti-Kir2.3 (KCNJ4) Antibody Cat #: APC-032
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EFGS HLDLE RMQAA TLPLD N, corresponding to amino acid residues 418-437 of rat Kir2.3 (Accession P52190). Intracellular, C-terminal part.
Homology
Mouse - identical; guinea pig - 19/20 amino acid residues identical; hamster, human, Xenopus - 18/20 amino acid residues identical.
Kir2.3/KCNJ4 Blocking Peptide Cat #: BLP-PC032
Size:
40 µg
Anti-GIRK1 (Kir3.1) Antibody Cat #: APC-005
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence LQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGPTRMEGNLPAKLRKMNSDRFT, corresponding to residues 437-501 of mouse GIRK1 (Accession P63250). Intracellular, C-terminus.
Homology
Rat - identical; human - 64/66 amino acid residues identical; guinea pig - 63/66 amino acid residues identical; chicken - 59/66 amino acid residues identical.
GIRK1/Kir3.1 Blocking Peptide Cat #: BLP-PC005
Size:
0.12 mg
Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody Cat #: ALM-031
Size:
25 µg
Host: Mouse
Type: Monoclonal
Application:
IC, IF, LCI, WB
Reactivity:
H, M
Immunogen
Synthetic peptide mapping to the extracellular loop of human GIRK1 (Accession P48549).
Anti-GIRK2 (Kir3.2) Antibody Cat #: APC-006
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2 (Accession P48542). Intracellular, C-terminal part.
Homology
Rat - 40/41 amino acid residues identical; golden hamster - 39/41 amino acid residues identical; human - 37/41 amino acid residues identical.
GIRK2/Kir3.2 Blocking Peptide Cat #: BLP-PC006
Size:
0.12 mg
Anti-GIRK3 (Kir3.3) Antibody Cat #: APC-038
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat KCNJ9 (Accession Q63511). Intracellular, C-terminus.
Homology
Mouse, human - identical.
GIRK3/Kir3.3 Blocking Peptide Cat #: BLP-PC038
Size:
40 µg
Anti-KCNJ5 (Kir3.4) Antibody Cat #: APC-027
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4 (Accession P48548). Intracellular, N-terminus.
Homology
Mouse, human, pig - identical.
KCNJ5/Kir3.4 Blocking Peptide Cat #: BLP-PC027
Size:
40 µg
Anti-Kir4.1 (KCNJ10) Antibody Cat #: APC-035
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KLEE SLREQ AEKEG SALSV R, corresponding to amino acid residues 356-375 of rat Kir4.1 (Accession P49655). Intracellular, C-terminus.
Homology
Human, mouse - identical.
Kir4.1/KCNJ10 Blocking Peptide Cat #: BLP-PC035
Size:
40 µg
Guinea pig Anti-Kir4.1 (KCNJ10) Antibody Cat #: APC-035-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KLEESLREQAEKEGSALSVR, corresponding to amino acid residues 356-375 of rat Kir4.1 (Accession P49655). Intracellular, C-terminus.
Homology
Human, mouse - identical.
Kir4.1/KCNJ10 Blocking Peptide Cat #: BLP-PC035
Size:
40 µg
Anti-Kir4.1 (KCNJ10) (extracellular) Antibody Cat #: APC-165
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1 (Accession P49655). Extracellular loop.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.
Kir4.1/KCNJ10 (extracellular) Blocking Peptide Cat #: BLP-PC165
Size:
40 µg
Anti-KCNJ15 (Kir4.2) Antibody Cat #: APC-058
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse KCNJ15 (Accession O88932). Intracellular, C-terminal part.
Homology
Guinea pig - 19/20 amino acid residues identical; human - 18/20 amino acid residues identical.
KCNJ15/Kir4.2 Blocking Peptide Cat #: BLP-PC058
Size:
40 µg
Anti-Kir5.1 Antibody Cat #: APC-123
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat KCNJ16 (Accession P52191). Intracellular, C-terminal.
Homology
Mouse - identical; human 14/15 amino acid residues-identical.
Kir5.1 Blocking Peptide Cat #: BLP-PC123
Size:
40 µg
Anti-Kir6.1 (KCNJ8) Antibody Cat #: APC-105
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat Kir6.1 (Accession Q63664). Intracellular, C-terminal part.
Homology
Mouse, human, pig, rabbit - 15/16 amino acid residues identical.
Kir6.1/KCNJ8 Blocking Peptide Cat #: BLP-PC105
Size:
40 µg
Anti-Kir6.2 Antibody Cat #: APC-020
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminal part.
Homology
Mouse - identical; human - 12/14 amino acid residues identical.
Kir6.2 Blocking Peptide Cat #: BLP-PC020
Size:
40 µg
Guinea pig Anti-Kir6.2 Antibody Cat #: APC-020-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminus.
Homology
Mouse - identical; human - 12/14 amino acid residues identical.
Kir6.2 Blocking Peptide Cat #: BLP-PC020
Size:
40 µg
Anti-Kir7.1 (extracellular) Antibody Cat #: APC-125
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IFC, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80-94 of rat KCNJ13 (Accession O70617). Extracellular loop.
Homology
Mouse - identical; human - 13/15 amino acid residues identical.
Kir7.1 (extracellular) Blocking Peptide Cat #: BLP-PC125
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
A mouse monoclonal antibody (#ALM-031) and guinea pig polyclonal antibodies (#APC-035-GP & #APC-020-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
