Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir2.1/KCNJ2 (extracellular) Blocking Peptide (#BLP-PC159) is the original antigen used for immunization during Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.1/KCNJ2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and mouse heart lysate (lanes 3 and 6):1-3. Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159), (1:200).
4-6. Anti-Kir2.1/KCNJ2 (extracellular) Antibody, preincubated with Kir2.1/KCNJ2 (extracellular) Blocking Peptide (#BLP-PC159).
Expression of Kir2.1 in mouse midbrain.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the dorsal midbrain region showed immunoreactivity (green) in multipolar neurons (vertical arrow). B. Pre-incubation of the antibody with Kir2.1/KCNJ2 (extracellular) Blocking Peptide (BLP-PC159), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DLDASKESKA(S)VSE, corresponding to amino acid residues 112 - 125 of rat Kir2.1 (Accession Q64273).
Accession (Uniprot) Number Q64273
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
