Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir2.3/KCNJ4 Blocking Peptide (#BLP-PC032) is the original antigen used for immunization during Anti-Kir2.3 (KCNJ4) Antibody (#APC-032) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.3/KCNJ4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:1,2. Anti-Kir2.3 (KCNJ4) Antibody (#APC-032), (1:200)
3,4. Anti-Kir2.3 (KCNJ4) Antibody, preincubated with Kir2.3/KCNJ4 Blocking Peptide (#BLP-PC032).- Rat brain sections.
Properties
Sequence
- (C)EFGS HLDLE RMQAA TLPLD N, corresponding to amino acid residues 418-437 of rat Kir2.3 (Accession P52190).
Accession (Uniprot) Number P52190
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
