Overview
Alomone Labs is pleased to offer the GIRK (Kir3) Channel Antibody Explorer Kit (#AK-227). This Explorer Kit includes antibodies targeting GIRK (Kir3) channels along with their respective peptide control antigen (where applicable). An ideal tool for screening purposes.
Compounds
| Product Name | Cat # | Size |
|---|---|---|
Anti-GIRK1 (Kir3.1) Antibody |
APC-005 | 1 x 50 µl |
GIRK1/Kir3.1 Blocking Peptide |
BLP-PC005 | 1 x 0.12 mg |
Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody |
ALM-031 | 1 x 25 µg |
Anti-GIRK2 (Kir3.2) Antibody |
APC-006 | 1 x 50 µl |
GIRK2/Kir3.2 Blocking Peptide |
BLP-PC006 | 1 x 0.12 mg |
Anti-GIRK3 (Kir3.3) Antibody |
APC-038 | 1 x 50 µl |
GIRK3/Kir3.3 Blocking Peptide |
BLP-PC038 | 1 x 40 µg |
Anti-KCNJ5 (Kir3.4) Antibody |
APC-027 | 1 x 50 µl |
KCNJ5/Kir3.4 Blocking Peptide |
BLP-PC027 | 1 x 40 µg |
Scientific Background
The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo or heterotetramers.
Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.
Kir3.1 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 (GIRK) family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel1,2.
- Dascal, N. (1997) Cell Signal 9, 551.
- Mark, M.D. et al. (2000) Eur. J. Biochem. 267, 5830.
