Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir4.1/KCNJ10 (extracellular) Blocking Peptide (#BLP-PC165) is the original antigen used for immunization during Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165) generation. The blocking peptide binds and ‘blocks’ Anti-Kir4.1/KCNJ10 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1, 2. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:500).
3, 4. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody, preincubated with Kir4.1/KCNJ10 (extracellular) Blocking Peptide (#BLP-PC165).Rat brain sections (1:200), (Cui, Y. et al. (2018) Nature 554, 323.).
Properties
Sequence
- (C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1 (Accession P49655).
Accession (Uniprot) Number P49655
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
