Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035) is the original antigen used for immunization during Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) generation. The blocking peptide binds and ‘blocks’ Anti-Kir4.1/KCNJ10 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Kir4.1 (KCNJ10) Antibody (#APC-035), (1:400).
2. Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).- Rat brain sections.
Mouse hippocampus (1:200) (Hsu, M.S. et al. (2011) Neuroscience 178, 21.).
Human brain tissue (Saadoun, S. et al. (2003) J. Clin. Pathol. 56, 972.). 
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6):1-3. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:800).
4-6. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).
Expression of Kir4.1 in rat hippocampal dentate gyrusImmunohistochemical staining of rat frozen brain sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:300). A. Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer (Mol). B. The same section was stained with mouse anti-glial fibrillary acidic protein (GFAP), (red) showing astrocyte profiles. C. Merge of Kir4.1 and GFAP images reveals colocalization on some branches of astrocytic fibers. In addition, Kir4.1 is expressed in the fine ramification of astrocytic fibers, thus creating a “halo-like” cloud around the GFAP astrocytic outline. DAPI (blue) counterstain reveals the outline of the dentate granule layer.
Properties
Sequence
- (C)KLEE SLREQ AEKEG SALSV R, corresponding to amino acid residues 356-375 of rat Kir4.1 (Accession P49655).
Accession (Uniprot) Number P49655
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
