Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KLEE SLREQ AEKEG SALSV R, corresponding to amino acid residues 356-375 of rat Kir4.1 (Accession P49655).
Accession (Uniprot) Number P49655
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Kir4.1 (KCNJ10) Antibody (#APC-035), (1:400).
2. Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).- Rat brain sections.
Mouse hippocampus (1:200) (Hsu, M.S. et al. (2011) Neuroscience 178, 21.).
Human brain tissue (Saadoun, S. et al. (2003) J. Clin. Pathol. 56, 972.). 
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6):1-3. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:800).
4-6. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).- Rat brain sections (1:300). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
