Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat KCNJ16 (Accession P52191).
Accession (Uniprot) Number P52191
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes:1,2. Anti-Kir5.1 Antibody (#APC-123), (1:200).
3,4. Anti-Kir5.1 Antibody, preincubated with Kir5.1 Blocking Peptide (#BLP-PC123).
Western blot analysis of mouse brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:1,2. Anti-Kir5.1 Antibody (#APC-123), (1:200).
3,4. Anti-Kir5.1 Antibody, preincubated with Kir5.1 Blocking Peptide (#BLP-PC123).- Rat kidney sections (paraffin embedded), (1:100).
Human brain sections (1:500) (Schirmer, L. et al. (2014) Ann. Neurol. 75, 810.).
Last update: 02/01/2024
