Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir6.2 Blocking Peptide (#BLP-PC020) is the original antigen used for immunization during Anti-Kir6.2 Antibody (#APC-020) generation. The blocking peptide binds and ‘blocks’ Anti-Kir6.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas membranes:1. Anti-Kir6.2 Antibody (#APC-020), (1:200).
2. Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
Expression of Kir6.2 in rat pancreasImmunohistochemical staining of rat pancreas using Anti-Kir6.2 Antibody (#APC-020). A. Strong granular staining in a number of cells within the Islets of Langerhans is readily detected (red). B. The negative control slide shows no staining.- Human placenta sections (Lybaert, P. et al. (2013) Placenta 34, 467.).
Western blot analysis of rat heart membranes (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):1-2. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:500).
3-4. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
Western blot analysis of human SHSY-5Y neuroblastoma cell lysate:1. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:200).
2. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
Expression of Kir6.2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody (red staining). Kir6.2 staining appears in the hilus region in neuronal outlines (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673).
Accession (Uniprot) Number P70673
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
