Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kir7.1 (extracellular) Blocking Peptide (#BLP-PC125) is the original antigen used for immunization during Anti-Kir7.1 (extracellular) Antibody (#APC-125) generation. The blocking peptide binds and ‘blocks’ Anti-Kir7.1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse kidney lysates (lanes 3 and 6):1-3. Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:200).
4-6. Anti-Kir7.1 (extracellular) Antibody, preincubated with Kir7.1 (extracellular) Blocking Peptide (#BLP-PC125).
Expression of Kir7.1 in rat kidneyImmunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:100). Staining is present in both distal (DT) and proximal (PT) tubules and in the collecting ducts (CD) in the renal cortex. Note that staining is absent both in glomeruli (Gl) and blood vessels (arrow). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80-94 of rat KCNJ13 (Accession O70617).
Accession (Uniprot) Number O70617
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
