Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kvβ2 Blocking Peptide (#BLP-PC117) is the original antigen used for immunization during Anti-KVβ2 Antibody (#APC-117) generation. The blocking peptide binds and ‘blocks’ Anti-Kvβ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and membranes (lanes 2 and 4):1,2. Anti-KVβ2 Antibody (#APC-117), (1:200).
3,4. Anti-KVβ2 Antibody, preincubated with Kvβ2 Blocking Peptide (#BLP-PC117).
Western blot analysis of human Jurkat T cells:1. Anti-KVβ2 Antibody (#APC-117), (1:200).
2. Anti-KVβ2 Antibody, preincubated with Kvβ2 Blocking Peptide (#BLP-PC117).
Expression of KVβ2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-KVβ2 Antibody (#APC-117). A. KVβ2 appears adjacent to Purkinje cells (green). B. Staining of GABAergic cells with mouse anti-parvalbumin (PV, red). C. Confocal merge of KVβ2 and PV demonstrates the presence of KVβ2 adjacent to Purkinje cells.
Properties
Sequence
- SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat KCNAB2 (Accession P62483).
Accession (Uniprot) Number P62483
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
