Cat #: ESP-501_PACK
Size: 10 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-KV1.3 (KCNA3) Antibody Cat #: APC-002
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence TLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV, corresponding to amino acid residues 523-575 of human KV1.3 (Accession P22001). Intracellular, C-terminus.
Homology
Rat, rabbit, mouse - identical.
Anti-KV1.3 (KCNA3) (extracellular) Antibody Cat #: APC-101
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
Homology
Rat, mouse - 12/14 amino acid residues identical.
Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody Cat #: APC-101-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). 1st extracellular loop.
Homology
Rat, mouse - 12/14 amino acid residues identical.
Pharmacological Reagents
Aa1 Toxin Cat #: RTA-400
Size:
10 µg
MW: 3868 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
100 nM - 1 µM.
Activity
Aa1 Toxin is a blocker of A-type voltage-gated transient K+ channels of cerebellar granular cells1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Agitoxin-2 Cat #: STA-420
Size:
0.1 mg
MW: 4091 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
50 pM - 10 nM.
Activity
Agitoxin-2 is a blocker of Shaker voltage-gated K+ channels as well as the mammalian homologues of Shaker.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two week at 4°C or three months at -20°C.
Hemitoxin Cat #: STH-500
Size:
50 µg
MW: 3898 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1 nM - 1 μM.
Activity
Hemitoxin reversibly inhibits rat voltage-gated K+ channels KV1.1 (KCNA1), KV1.2 (KCNA2) and KV1.3 (KCNA3)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Kaliotoxin-1 Cat #: STK-370
Size:
0.1 mg
MW: 4149 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
Kaliotoxin-1 is a potent inhibitor of large conductance Ca2+-activated K+ channels (BKCa)1,2. Kaliotoxin-1 also binds the dendrotoxin sensitive voltage-dependent K+ channels (KV1.1, KV1.2 and KV1.3 nM)3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#APC-101-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
