Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101) is the original antigen used for immunization during Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.3/KCNA3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:500).
2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
Western blot analysis of human Jurkat T cells:1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).- Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:200).
3,4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
Expression of KV1.3 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:100). KV1.3 staining (red) is detected in Bergmann glia outlines (arrow). DAPI is used as the counterstain (blue) .
Properties
Sequence
- KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001).
Accession (Uniprot) Number P22001
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
