Overview
Type: GST fusion protein
Form: Lyophilized powder
Kv1.5/KCNA5 Blocking Peptide (#BLP-PC004) is the original antigen used for immunization during Anti-KV1.5 (KCNA5) Antibody (#APC-004) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.5/KCNA5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV1.5 (KCNA5) Antibody (#APC-004), (1:100).
2. Anti-KV1.5 (KCNA5) Antibody, preincubated with Kv1.5/KCNA5 Blocking Peptide (#BLP-PC004).- Rat brain sections (see also publications using this product).
Mouse heart sections (1 µg/ml) (Roepke, T.K. et al. (2008) FASEB J 22, 3648.).
Properties
Sequence
- GST fusion protein with the sequence HRETDHEEQAALKEEQGIQRRESGLDTGGQRKVSCSKASFHKTGGPLEST DSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTS RETDL, corresponding to amino acid residues 513-602 of mouse KV1.5 (Accession Q61762).
Accession (Uniprot) Number Q61762
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 1.2 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg fusion protein per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
