Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1 (Accession P15387).
Accession (Uniprot) Number P15387
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV2.1 Antibody (#APC-012), (1:500).
2. Anti-KV2.1 Antibody, preincubated with KV2.1 Blocking Peptide (BLP-PC012).- Rat pancreas (1:50).
Mouse pancreas sections (10 μg/ml) (MacDonald, P.E. et al. (2002) J. Biol. Chem. 277, 44938.).
Human brain sections (1:500) (Mezey, E. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 1364.). 
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Guinea pig Anti-KV2.1 Antibody (#APC-012-GP), (1:500).
3,4. Guinea pig Anti-KV2.1 Antibody, preincubated with Kv2.1 Blocking Peptide (#BLP-PC012).- Rat brain sections (1:400).
Last update: 02/01/2024
