Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv2.2/KCNB2 Blocking Peptide (#BLP-PC120) is the original antigen used for immunization during Anti-KV2.2 (KCNB2) Antibody (#APC-120) generation. The blocking peptide binds and ‘blocks’ Anti-Kv2.2/KCNB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-KV2.2 (KCNB2) Antibody (#APC-120), (1:2000).
2. Anti-KV2.2 (KCNB2) Antibody, preincubated with Kv2.2/KCNB2 Blocking Peptide (#BLP-PC120).- Rat brain sections (Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.).
Properties
Sequence
- CRQDIYQAVGEVKKD, corresponding to amino acid residues 859-873 of rat KV2.2 (Accession Q63099).
Accession (Uniprot) Number Q63099
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
