Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv3.2/KCNC2 Blocking Peptide (#BLP-PC011) is the original antigen used for immunization during Anti-KV3.2 (KCNC2) Antibody (#APC-011) generation. The blocking peptide binds and ‘blocks’ Anti-Kv3.2/KCNC2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV3.2 (KCNC2) Antibody (#APC-011), (1:200).
2. Anti-KV3.2 (KCNC2) Antibody, preincubated with Kv3.2/KCNC2 Blocking Peptide (#BLP-PC011).- Rat brain sections. Human pancreatic islets (Yan, L. et al. (2004) Diabetes 53, 597.).
Properties
Sequence
- DLGGKRLGIEDAAGLGGPDGK(C), corresponding to amino acid residues 184-204 of rat KV3.2 (Accession P22462).
Accession (Uniprot) Number P22462
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
