Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv4.1/KCND1 Blocking Peptide (#BLP-PC119) is the original antigen used for immunization during Anti-KV4.1 (KCND1) Antibody (#APC-119) generation. The blocking peptide binds and ‘blocks’ Anti-Kv4.1/KCND1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-KV4.1 (KCND1) Antibody (#APC-119), (1:400).
2. Anti- KV4.1 (KCND1) Antibody, preincubated with Kv4.1/KCND1 Blocking Peptide (#BLP-PC119).
Western blot analysis of human neuroblastoma cell line SH-SY5Y (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-KV4.1 (KCND1) Antibody (#APC-119), (1:400).
3,4. Anti-KV4.1 (KCND1) Antibody, preincubated with Kv4.1/KCND1 Blocking Peptide (#BLP-PC119).- Human breast cancer tissue (1:200) (Jang, S.H. et al. (2009) Biochem. Biophys. Res. Commun. 384, 180.).
Properties
Sequence
- (C)KRRAIRLANSTAS, corresponding to amino acid residues 538-550 of human KCND1 (Accession Q9NSA2).
Accession (Uniprot) Number Q9NSA2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
