Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv4.3 Blocking Peptide (#BLP-PC017) is the original antigen used for immunization during Anti-KV4.3 Antibody (#APC-017) generation. The blocking peptide binds and ‘blocks’ Anti-Kv4.3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV4.3 Antibody (#APC-017), (1:200).
2. Anti-KV4.3 Antibody, preincubated with Kv4.3 Blocking Peptide (#BLP-PC017).
Expression of KV4.3 in rat hippocampal CA1 regionImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV4.3 Antibody (#APC-017), (1:400), followed by donkey anti-rabbit-Cy3 antibody (red). Staining appears in neurons of the CA1 hippocampal region (arrow). Nuclei are stained with DAPI (blue).- Mouse colon lysate (1:100) (Amberg, G.C. et al. (2002) J. Physiol. 544.2, 403.).
Properties
Sequence
- (C)NEALELTGTPEEEHMGK, corresponding to amino acid residues 451-468 of human KV4.3 (Accession O60577).
Accession (Uniprot) Number O60577
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
